Figure 4: Volumetric quantification of cellular biochemical components in a 3D culture system. | Nature Communications

Figure 4: Volumetric quantification of cellular biochemical components in a 3D culture system.

From: Quantitative volumetric Raman imaging of three dimensional cell cultures

Figure 4

Representative 3D reconstructions by qVRI, of the main subcellular components of hMSCs (n=2 cells per hydrogel) in (a) a bioactive (PEG-MMP+RGD) and a bioinert (PEG) hydrogel, and their corresponding (b) endmember Raman spectra from VCA (showing five components); from top to bottom cytoplasm (blue), nucleus (red), triacylglycerols (green), phospholipids (orange) and hydrogel (cyan). (c) Bar chart of mean abundance values for each subcellular component. Error bars represent one standard deviation around the mean. Scale bar, 10 μm.

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