Figure 5: Joint-specific histone marks in HOXA and HOXC clusters in SFs. | Nature Communications

Figure 5: Joint-specific histone marks in HOXA and HOXC clusters in SFs.

From: Epigenetically-driven anatomical diversity of synovial fibroblasts guides joint-specific fibroblast functions

Figure 5

(a) Regulation of the positional HOX signatures in knee (blue), shoulder (grey) and hand (orange) SFs by histone modifications. H3K27me3, H3K4me3 and H3K27ac marks were measured by chromatin immunoprecipitation DNA sequencing (ChIP-seq) and significant enrichment calculated by the peak calling algorithm SICER. Data are visualized using Integrative Genomics Viewer (IGV) (b). Joint-specific effects of I-BET151, an inhibitor of BET reader proteins, on the expression of HOXA and C transcripts in hand (n=4), shoulder (n=3) and knee (n=3) RA SFs (x-fold calculations; expression in hand and knee RA SFs, respectively, was set to 1). Statistics: one-sample t-test or two-tailed paired t-test, *P<0.05; **P<0.01.

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