Figure 1: Loss of Par3 induces apoptosis.
From: The Par3 polarity protein is an exocyst receptor essential for mammary cell survival
(a,b) Knockdown of Par3 in NMuMG or Eph4 cells induces cleaved Caspase-3 activation. The experiments were repeated at least three times and representative blots are shown. (c) Knockdown of Par3 in NMuMG cells induces Parp cleavage. (d) Immunofluorescence staining of NMuMG cells showing the activity of Caspases 3 and 7 in NMuMG cells using CellEvent Caspase-3/7 Green ReadyProbes (green) and phalloidin Alexa594 (red) and Hoechst 33342 (blue). Scale bars, 50 μm. (e) Quantification of cleaved Caspase-3 staining in NMuMG cells. Data represented as the mean percentage of cleaved Caspase-3 compared with control, the graph shows mean±s.e.m. for four experimental replicates and P value was calculated by the Student’s t-test. (f) Phase contrast images of NMuMG cells treated with shLuc, shPar3 or shPar3+YFP-hPAR3b. Scale bars, 200 μm. (g) Immunoblot showing cleaved Caspase-3 induction by Par3 depletion can be reversed by expression of YFP-hPAR3b. (h) Co-culture of NMuMG cells expressing shLuc plus GFP (green) or shPar3 plus RFP (red) and stained with Hoechst 33342 (blue). The experiment was repeated three times and a representative image is shown. The boxed region is shown as magnified below. Scale bar, 100 μm. (i) Quantification of cells with normal or condensed nuclear morphology in the experiment shown in h. Error bars represent mean±s.e.m.
