Figure 5: E-cadherin co-localizes with Rab11-positive vesicles.
From: The Par3 polarity protein is an exocyst receptor essential for mammary cell survival
NMuMG and Eph4 cells expressing (a) YFP-Rab11, or (b) YFP-Rab8 were transduced with shLuc or shPar3, fixed and stained for E-cadherin. Fluorescent intensity profiles taken over a 20–30 μm straight line for both E-cadherin and YFP-Rab11 and overlaid. Confocal images were taken sequentially using 488 and 641 nm excitation lasers. White dashed boxed ROIs are magnified and arrows direct to objects where structures in both channels overlap. Scale bars, 20 μm.
