Figure 2: IR and IGF1R modulate different signalling upon hormonal stimulation.
From: Domain-dependent effects of insulin and IGF-1 receptors on signalling and gene expression
(a) Immunoblotting of protein phosphorylation in lysates from cells stimulated with insulin or IGF-1 (10 nM) for 5 min. (b–d) Densitometric analysis of phosphorylated proteins following 5 min after stimulation. Data are mean±s.e.m. (*P<0.05; **P<0.01. One-way ANOVA followed by Newman–Keuls post-hoc analysis; three clones used in three independent experiments). (e) Immunoblotting of protein phosphorylation in lysates from cells stimulated with insulin or IGF-1 (10 nM) for 15 min. (f–i) Densitometry analysis of phosphorylated proteins following 15 min ligand stimulation. Data are mean±s.e.m. (*P<0.05; **P<0.01. One-way ANOVA followed by Newman–Keuls post-hoc analysis; three clones used in three independent experiments).
