Figure 4: Differential roles of IR-ECD and IGF1R-ECD in regulating gene expression.
From: Domain-dependent effects of insulin and IGF-1 receptors on signalling and gene expression
(a) Scattered plot showing distribution of log fold change of each probeset’s expression after stimulation in cells expressing receptors with IR-ECD (y axis) versus that in cells expressing receptors with IGF1R-ECD (x axis). Each dot represents the mean of all the clones expressing the receptors with the same ECD. The highlighted dots represent the probe sets with fold-change difference between IR-ECD and IGF1R-ECD over 50%. (b) Heat map of top 20 significant genes in both groups in a. (c) Fold changes of expression in response to stimulation for representative genes highly induced in cells expressing receptors with IR-ECD were confirmed by qPCR versus TBP. (d) Fold changes of expression in response to stimulation for representative genes highly suppressed in cells expressing receptors with IR-ECD were confirmed by qPCR versus TBP. Data are mean±s.e.m. (*P<0.05; **P<0.01; ***P<0.001. One-way ANOVA followed by Newman–Keuls post-hoc analysis, n=6).
