Figure 7: Leu973 is a key residue differentiating IR signalling from IGF1R signalling.
From: Domain-dependent effects of insulin and IGF-1 receptors on signalling and gene expression
(a) Immunoblotting of the phosphorylation of IR, IRS-1 and Akt in lysates from preadipocytes expressing normal IR, IRY972F and IRL973F stimulated with 100 nM insulin for 5 min. (b–d) Densitometric analysis of phosphorylated IR, IRS-1 and Akt following 5 min stimulation. Data are mean±s.e.m. (*P<0.05; **P<0.01. One-way ANOVA followed by t-test with Bonferroni correction. n=3). (e) Immunoblotting of the phosphorylation of Shc, Gab-1, ERK, p70S6K1 and S6 in lysates from preadipocytes expressing normal IR, IRY972F and IRL973F stimulated with 100 nM insulin for 5 min. (f–j) Densitometric analysis of phosphorylated Shc, Gab-1, ERK, p70S6K1 and S6 following 5 min stimulation. Data are mean±s.e.m. (*P<0.05; **P<0.01. One-way ANOVA followed by t-test with Bonferroni correction. n=3). (k,l) Fold changes of expression in response to stimulation for representative genes regulated by normal IR or IRL973F were confirmed by qPCR with TBP as the housekeeping gene. Data are presented as mean±s.e.m. (*P<0.05; **P<0.01; ***P<0.001. Student t-test. n=3).
