Figure 1: LOX regulates EGFR activation and cell surface retention.
From: Lysyl oxidase drives tumour progression by trapping EGF receptors at the cell surface
(a) Protein phosphorylation changes in LOX-depleted MDA-MB-231 cells on 2D plastic and (b) in 3D collagen gels as shown by a RTK antibody array (±20% arbitrary cut-off). (c) Western blots of LOX, pY1068 EGFR, surface EGFR, total EGFR and GAPDH in control (shCtl) or LOX-depleted (shLOX A,B) MDA-MB-231 and U87 cells. (d) Quantification of LOX protein expression, (e) EGFR activation and (f) surface EGFR level in MDA-MB-231 and U87 cells from experiments in c. All data are represented as mean±s.d. from three independent experiments. **P<0.01, Student’s t-test. (g,h) Levels of EGFR activation and (i,j) surface EGFR in MDA-MB-231 or U87 control (shCtl) or LOX-depleted (shLOX A) cells following EGF stimulation for indicated time. (k,l) AKT activation in MDA-MB-231 or U87 control (shCtl) or LOX-depleted (shLOX A,B) cells following EGF stimulation for indicated time. All data in g–l are represented as mean±s.d. from three independent experiments at each indicated time point. **P<0.01, Student’s t-test.
