Figure 5: CF-CHP binding to fatigue loaded fascicles. | Nature Communications

Figure 5: CF-CHP binding to fatigue loaded fascicles.

From: Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides

Figure 5

(a) Mean fluorescence intensity versus fatigue loading condition for samples loaded between 1 and 5% strain at 0.1 and 1.0 Hz (n=3, mean±s.e.m.). Statistically significant intensity increase from the controls was observed in 0.1 Hz samples after 100 cycles and in 1.0 Hz samples after 1,000 cycles. (b) Representative wide-field fluorescence images reveal different CHP staining patterns between the 0.1 and 1.0 Hz samples. The staining images help explain the apparent overall high intensity of 0.1 Hz samples, as CHP was bound to a larger area in 0.1 Hz samples compared to 1.0 Hz samples tested for the same number of cycles. Arrows indicate approximate location of clamps. Scale bars, 2 mm.

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