Figure 3: Functional and clinical validation of identified driver TFs. | Nature Communications

Figure 3: Functional and clinical validation of identified driver TFs.

From: Computational identification of mutually exclusive transcriptional drivers dysregulating metastatic microRNAs in prostate cancer

Figure 3

(a) Venn diagram showing the overlap between experimentally validated microRNAs co-regulated by AR, HOXC6 and NKX2-2 and microRNAs differentially expressed in metastatic prostate cancers derived from two independent data sets. (b) The set of microRNAs co-regulated by AR, HOXC6 and NKX2-2 target genes in key metastatic pathways. Circles represent genes and squares represent microRNAs. The node colours represented the annotated function of the gene (for circles) and the expression change of the microRNA (for squares). (c) Representative images showing the results of the migration assay. Pictures in the top row are RWPE-1 cells overexpressing AR, HOXC6, NKX2-2 or a control empty vector treated with control microRNA mimics. Pictures in the bottom row are cells treated with miR-133a mimics. Scale bars, 100 μm. The number of migrated cells were quantified and illustrated in the bar chart. *P<0.01, as determined using the two-tailed Student’s t-test. (d) Same as c for the invasion assay. (e) Kaplan–Meier survival analyses based on the overexpression of AR, HOXC6 or NKX2-2 in three independent prostate cancer data sets. Results shown are representative of three independent experiments.

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