Figure 1: Overexpression of MYC moderately increases REV-ERBα in U2OS cells. | Nature Communications

Figure 1: Overexpression of MYC moderately increases REV-ERBα in U2OS cells.

From: Correspondence: Reply to ‘Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα’

Figure 1

(a) qPCR reevaluation of RNA expression data from Shostak et al.1 at two circadian time points using primers from Zhang et al.5 and Altman et al.2. Data were normalized to GAPDH (upper row) or B2M (lower row) by ΔΔCt (n=3). (b) qPCR analysis of circadian expression profiles of indicated transcripts in synchronized U2OS t-rex tetO-MYC cells normalized to GAPDH (upper row) or B2M (lower row) (n=3). qPCR products were detected with SYBR Green, corresponding quantification with TaqMan probes is shown in Supplementary Fig. 1a. (c) Western blot analysis (upper panel) and densitometric protein quantification (lower panel) of indicated proteins in synchronized U2OS t-rex tetO-MYC cells in presence (Dox) and absence (PBS) of overexpressed MYC (n=1). Data are presented as mean±s.e.m. *P<0.05; two-way ANOVA with Bonferroni post-test.

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