Figure 2: MYC represses BMAL1 and CLOCK in absence of REV-ERBα. | Nature Communications

Figure 2: MYC represses BMAL1 and CLOCK in absence of REV-ERBα.

From: Correspondence: Reply to ‘Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα’

Figure 2

(a) MYC-dependent reduction of BMAL1 and CLOCK levels in presence and absence of REV-ERBα. U2OS t-rex tetO-MYC cells were transfected with REV-ERBα or negative siRNAs as indicated. After 24 h cells were treated with doxycycline to induce MYC (MYC ox) or with PBS for control (Ctrl). Western blot analysis (left) and densitometric protein quantification (right) (n=3) are shown. *P<0.05 for Ctrl versus MYC ox; #P<0.05 for neg siRNA versus REV-ERBα siRNA by two-way ANOVA. (b) MYC-dependent repression of Bmal1 promoters with and without ROREs. Schematic of the Bmal1-luc construct showing the location of ROREs. Sequence changes inactivating ROREs6,7 are underlined (right panel). U2OS t-rex tetO-MYC cells transiently transfected with Bmal1-luc (left) or Bmal1-ΔRORE1,2-luc (right) were treated with doxycycline (MYC ox) or PBS (Ctrl) as indicated and, bioluminescence traces were recorded (n=3). Data are presented as mean±s.e.m. (c) Schematic of MYC-dependent direct and indirect repression of BMAL1.

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