Figure 5: Chromatin landscapes of ADC and SCCs reveal de-repression of squamous genes.
From: Lkb1 inactivation drives lung cancer lineage switching governed by Polycomb Repressive Complex 2
(a) Heatmap depicting global analysis of H3K4me3 marked chromatin in ADC and SCC from KRAS/Lkb1 mice. (b) Genome browser snapshots of the squamous loci Sox2, Ngfr and Krt6b/6a/5 with the indicated ChIP-sequencing peaks for H3K27ac, H3K27me3 and H3K4me3 in the ADC (blue) and SCC (red) KRAS/Lkb1 tumour samples. (c) GREAT analysis of genes adjacent to H3K4me3-enriched sites specific to SCC showed that this gene set is enriched for genes (from MSigDB) that are normally repressed by Polycomb in other cell types. Graphed as -log10 (P value represents hypergeometric probability test.) Level of H3K27me3 marks at all loci enriched for H3K27ac (black), loci with H3K27ac unique to ADC (blue) or loci with H3K27ac unique to SCC (red) in either ADC (left panel) or SCC (right panel) from KRAS/Lkb1 mice. See also Supplementary Fig. 5a–c.
