Figure 5: POT1–TPP1 telomeric DNA binding assays.
From: Structural and functional analysis of the human POT1-TPP1 telomeric complex
(a) FP assays of POT1C and the POT1C–TPP1(PBD) complex with a single-stranded DNA probe consisting of 3 telomeric repeats (18mer). (b) FP assays of the WT and mutant (those that partially disrupt the POT1–TPP1 complex P446Q, C591W and Q623H) flPOT1 with the 18mer DNA probe. (c) FP assays of the WT and mutant flPOT1−TPP1(87) complex with the 18mer. (d) Bar graph showing the differences in Kd (nM) between the WT and mutant flPOT1 and flPOT1–TPP1(87) complex. The values are the average of three independent measurements and a two-tailed Student’s t-test was performed with respect to WT POT1–TPP1(PBD) complex: *P<0.05, **P<0.01.
