Figure 6: Cell imaging for localization of WT and mutant POT1 to telomeres. | Nature Communications

Figure 6: Cell imaging for localization of WT and mutant POT1 to telomeres.

From: Structural and functional analysis of the human POT1-TPP1 telomeric complex

Figure 6

(a) Western blot showing the levels of YFP tagged, WT and mutant (L343F, P466Q, P475L, R477T, A532P, I535F, C591W and Q623H) full length POT1. We used GFP (αGFP) or POT1 (αPOT1) to detect the levels of YFP-POT1 in each cell line. The band at 71 kDa and below the YFP-aPOT1 is a non specific protein. GAPDH was used as a loading control. (b) Maximum projection images of co-localization of YFP-POT1 (green) and Cherry-TRF2 (red) proteins are shown. Merged images include DAPI. Scale bar, 5 μm. The data clearly shows co-localization of WT and mutant flPOT1 proteins to telomeres.

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