Figure 6: Different RT–PCR patterns resulting from RNA of different topologies. | Nature Communications

Figure 6: Different RT–PCR patterns resulting from RNA of different topologies.

From: Synthesizing topological structures containing RNA

Figure 6

(a) Schematics illustrating topological blockage of RT. On knotted ssRNA template, RT can only extend the primer to a certain point, when the RT is stalled by the force imposed by the resistance to the bending of ds RNA–DNA hybrid and the tightening of ssRNA region. On circular ssRNA template, tandem-repeat cDNA product is generated. RNA templates are shown in blue, and DNA primer and cDNA products are in red. (b) RT reactions of TKj (lanes 1 and 2) and Cj (lanes 3 and 4) analysed by dPAGE. Samples in lanes 2 and 4 were treated by RNase H. Lane 5 contains TKj and Cj as references. (c) PCR reactions of the cDNA products from TKj (lane 1) and Cj (lane 2) analysed by dPAGE.

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