Figure 6: SpdCas9-assisted gene editing by the type II-C Cas9 from Neisseria cinerea (NcCas9). | Nature Communications

Figure 6: SpdCas9-assisted gene editing by the type II-C Cas9 from Neisseria cinerea (NcCas9).

From: Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting

Figure 6

(a) NcCas9 and SpdCas9 targets in the human POR locus. Targets are indicated by bars and PAMs are highlighted in purple (SpdCas9) and dark blue (NcCas9). (b) NcCas9 target cleavage activities (% indels) with or without the assistance of SpdCas9 binding at proximal locations. The sgRNA numbers correspond to the target numbers in a. M, wide-range DNA markers; ND, not determined.

Back to article page