Figure 7: SpdCas9-assisted gene editing by the type V Cpf1 from Francisella novicida (FnCpf1) in K562 cells. | Nature Communications

Figure 7: SpdCas9-assisted gene editing by the type V Cpf1 from Francisella novicida (FnCpf1) in K562 cells.

From: Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting

Figure 7

(a) FnCpf1 and SpdCas9 targets in the human POR locus. Targets are indicated by bars and PAMs are highlighted in purple (SpdCas9) and dark blue (FnCpf1). (b) FnCpf1 target cleavage activities (% indels) with or without SpdCas9 binding at proximal locations. The Fn-crRNA and Sp-sgRNA numbers correspond to the target numbers in a. Data are representatives of three independent experiments. M, wide-range DNA markers; ND, not determined.

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