Figure 5: Structural basis for slow product release.
From: A domain in human EXOG converts apoptotic endonuclease to DNA-repair exonuclease
(a) Electrostatic interactions of R314 and K148 with the 5′-P of the substrate strand. (b) Time-dependent nucleolytic reaction of hEXOG R314A(monomer) (150 nM) on 3′-F dsDNA (200 nM). (c) Quantification of digestion. Data represent the mean±s.e.m. of three independent experiments.
