Figure 7: IC87114-induced vesicle translocation is correlated with F-actin reorganization.

(a) TIRF images (left) and kymographs (right) of bovine chromaffin cells co-expressing Lifeact–GFP and NPY–Cherry treated with IC87114 (1 μM). The scanning lines (white arrows) indicate the position and direction of scan. (b) Time-course variation of Lifeact–GFP and NPY–Cherry fluorescence intensity in response to IC87114 treatment (n=4 regions of interest). The arrow indicates the timing of IC87114 addition. (c) Time-lapse images (left) of selected regions highlighting growing actin filaments and associated vesicle translocation before and during IC87114 treatment. Lifeact–GFP staining was filtered with a Laplacian 2D to highlight edges of actin filaments (middle panel). The timing (in s) after IC87114 treatment is indicated in the series of images. Scale bar, 1 μm. Kymographs (right) illustrate the changes in Lifeact–GFP and NPY–Cherry distribution on the scanning lines (red arrows). Note the increase in NPY–Cherry vesicle intensity occurring near the newly formed actin track.