Figure 5: Hmga1 expands the Paneth cell niche.

(a) IHC for lysozyme is shown from small intestines of WT and Hmga1 transgenic mice. Scale bar, 20 μm. (b) Lysozyme IHC pixels (mean±s.d.; imagine pro-plus 6.0 software) is shown in small intestinal cross-sections (n=78 crypts per group; 3 mice per genotype). **P<0.01; Mann–Whitney test. (c) Paneth cell number (mean±s.d.) per crypt (n=100 crypts per group; 3 mice per genotype). **P<0.00001; Mann-Whitney test. (d) Immunofluorescent co-staining for lysozyme, Ep-CAM and DAPI is shown from small intestines of WT and Hmga1 transgenic mice. Scale bars, 20 μm. (e) Paneth cell frequency (mean±s.d.) was obtained by dividing the Paneth cell number by total cell number per crypt in WT and Hmga1 intestine (n=50 crypts per group; 3 mice per genotype). **P<0.00001; Mann–Whitney test. (f) Granular cells in WT and Hmga1 organoid buds are shown via phase contrast microscopy. Scale bars, 50 μm. (g) Granular cells per bud PSA (mean±s.d.) from WT and Hmga1 organoids are shown (n=35 buds per group). **P<0.00001; Mann–Whitney test. (h) IHC for lysozyme (top) and immunofluorescent co-staining for lysozyme, Ep-CAM and DAPI (bottom) are shown in organoids from WT or Hmga1 mice. Scale bars, 50 μm. (i) Paneth cell frequency per bud (mean±s.d.) was calculated by dividing the Paneth cell number by the total bud cell number (n=30 organoids per group). **P<0.00001; Mann–Whitney test. (j) Relative expression (mean±s.d.) of Defcr-rs, the Paneth cell-specific transcript, is shown from two experiments performed in triplicate from organoids from WT and Hmga1 mice. Gapdh was used to control for loading. **P<0.01; two-tailed Student’s t-test.