Figure 1: Differentiation of macrophages from human iPSCs.

(a) Schematic diagram of the method of in vitro differentiation from human iPSCs to macrophages, with accompanying representative light microscopy images for each stage. Scale bars, 200 μm. (b) EM images of a human iPSdM (left) and a human blood monocyte differentiated macrophage (right). (c) Flow cytometry analyses for the expression of macrophage markers (CD11b, CD14, CD16, CD68) and pluripotency markers (SSEA4, OCT3/4) on human iPSdMs. Red lines represent cells stained with control isotype and blue lines represent cells stained with the relevant antibody.