Figure 5: Identification of HGF as a direct target of miR-26a/b.
From: Exosome-delivered EGFR regulates liver microenvironment to promote gastric cancer liver metastasis
(a) Predicted binding sites of miR-26a/b within the 3′-UTR of HGF mRNA. (b) Direct recognition of HGF 3′-UTR by miR-26a and miR-26b. Primary liver cells were co-transfected with firefly luciferase reporters containing either wild-type or mutant (mut) HGF 3′-UTR with miR-26a/b mimics, inhibitors and the corresponding normal control. The relative luciferase levels were detected using a luciferase kit at 24–36 h after transfection (n=3). (c) Western blot analysis of HGF expression in primary liver cells with the overexpression or suppression of miR-26a or miR-26b (n=3). (d) Relative levels of HGF mRNA in liver cells transfected with miR-26a/b mimics or inhibitors (n=3). The data represent the mean±s.e.m. **P<0.01 (Student’s t-test).
