Figure 6: Upregulated liver paracrine HGF promotes proliferation and invasion of SGC7901 cells.

Pretreated mixed liver cells were co-cultured with SGC7901 cells using a 0.4 μm membrane and the biology behaviour of SGC7901 cells were measured subsequently. (a) Schematic representation of the in vitro model for cell co-culture. (b) Western blot analysis of HGF expression in primary liver cells treated with HGF shRNA and HGF-overexpressing lentivirus (OE.HGF) (n=3). (c) Effects of SGC exosomes and control exosomes on the HGF secretion from mixed liver cells (n=3). (d) ELISA analysis of HGF secretion from primary liver cells treated with HGF siRNA or OE.HGF lentivirus (n=3). (e–g). Upregulated liver HGF promotes growth (e) and invasion (g) of SGC7901 cells (n=3). (h) c-MET and p-c-MET expression in mixed liver cells treated as above (n=3). The data represent the mean±s.e.m. *P<0.05, **P<0.01 (Student’s t-test).