Figure 7: In vivo verification for exosome-EGFR activates liver HGF by inhibiting miR-26a/b.
From: Exosome-delivered EGFR regulates liver microenvironment to promote gastric cancer liver metastasis
The livers of mice were pre-treated with HGF shRNA or HGF overexpressing lentivirus by multi-point injection, followed by orthotopic tumour implantation on the 6th day; finally, mice were killed and data were analysed on the 66th day. (a) A flow chart depicting the in vivo experimental design. (b) Immunohistochemistry (IHC) analysis of EGFR expression in primary gastric tumour. (c) Western blotting analysis of exosome EGFR in the serum of tumour-implanted mice (n=30). (d) Levels of HGF in liver tissues of GC tumour-implanted mice (n=30). (e) Electron-microscope scanning of exosomes isolated from mouse serum. (f) Relative levels of miR-26a/b in mouse liver tissues (n=30). The data represent the mean±s.e.m. **P<0.01 (Student’s t-test).
