Figure 8: Loss of FAF1 correlates with increased TβRII as well as downstream p-SMAD2 and p-AKT expression in patients. | Nature Communications

Figure 8: Loss of FAF1 correlates with increased TβRII as well as downstream p-SMAD2 and p-AKT expression in patients.

From: FAF1 phosphorylation by AKT accumulates TGF-β type II receptor and drives breast cancer metastasis

Figure 8

(a) Bioluminescent imaging (BLI) of representative mice from each group at week 3 following injection with empty vector (PLKO.1) or 4T1 breast cancer cells stably depleted of FAF1 and pre-treated with or without EGF (5 ng ml−1) for 8 h. Ventral images of the mice are shown (left panel). The BLI signal from primary tumours obtained from every mouse in each experimental group is shown. NS, not significant (right panel). (b) BLI of representative metastatic nodules in the lung from each experimental group is shown (left panel). Lung metastasis BLI signal for every mouse in each experimental group was analysed and is shown in the right panel (right panel). Data are presented as the mean±s.d. (cf) Immunohistochemistry analysis of FAF1, TβRII, p-SMAD2 and P-AKT 473 in breast cancer tissue microarrays; expression of FAF1 and TβRII, P-SMAD2 and P-AKT 473 in normal (n=11) and breast cancer (n=110) tissues. The data are the mean±s.e.m. P values obtained using Student’s t-tests are indicated (c). Representative images of each antibody staining are shown. Left panel: objective, × 5; right panel: objective, × 25 (d). Percentage of specimens displaying low or high FAF1 expression compared with the expression levels of TβRII, P-SMAD2 and P-AKT (e). Scatterplot showing the positive correlation between TβRII and P-AKT 473 expression in patients. Pearson’s coefficient tests were performed to assess significance (f).

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