Figure 3: Characterization of AP firing.

(a) Example traces representing four AP firing patterns used for characterization. Scale bar, 20 mV, 100 ms (b) Distribution of AP firing patterns for control (CTR; 4 subjects; n>250 at each time point), AS (3 subjects; n>250 at each time point) and UBE3A KO (1 line; n>40 at each time point) neurons at three developmental time bins. *P<0.0001 for differences between control and AS; χ² test. ^#P<0.0001 for differences between control and UBE3A KO; χ test. (c) AP firing distributions for (top to bottom) control neurons treated with UBE3A ASOs at 6 weeks in culture (7 coverslips, 15 cells per coverslip), control neurons treated with UBE3A ASOs at 18 weeks in culture (4 coverslips, 15 cells per coverslip), AS neurons treated with 1 μM topotecan (2–4 coverslips, 15 cells per coverslip), KCl (10 mM) treated control neurons from four control subjects (n=15 cells per coverslip for 1 coverslip for both vehicle and KCl for each control line). *P<0.0001, χ² test. (d) Example calcium imaging traces of spontaneous activity from control, AS and UBE3A KO cultures; scale bar, 30 s. (e) Quantification of number of calcium transient for control, AS and UBE3A KO cultures (n>60 at each time point for all three genotypes. *P<0.05 (Student’s t-test) for differences between control and AS. ^#P<0.05 (Student’s t-test) for differences between control and UBE3A KO. (f) AP amplitude (left), FWHM (middle) and AP threshold (right) for control and AS cultures at three time points (n>250 for both genotypes at each time point). *P<0.0001 for significant differences between control and AS (two-way analysis of variance (ANOVA)). Error bars represent mean ±s.e.m.