Figure 5: Synaptic plasticity in iPSC-derived neurons.
From: Disrupted neuronal maturation in Angelman syndrome-derived induced pluripotent stem cells
(a) Example traces of spontaneous excitatory synaptic currents from control (CTR; top), AS (middle) and UBE3A KO (bottom) neurons at (left to right) baseline, induction, 20–30 min post induction, and 50–60 min postinduction (see Methods for details). Scale bar for top CTR, AS and UBE3A KO, 20 pA, 1 s. Scale bar for bottom CTR trace, 10 pA, 200 ms. (b) Example time courses for frequency and amplitude of spontaneous synaptic currents from an individual neuron from (left) a CTR subject and (right) an AS patient. Plasticity induction period indicated by black bar. (c) Individual data for CTR and AS-derived neurons (12–25 weeks in culture) showing frequency of spontaneous synaptic currents during plasticity induction and 20–30 and 50–60 min post induction. *P<0.05, indicates difference for CTR or AS; #for UBE3A KO (Student’s t-test). (c,d) Group data for CTR (n=30), AS-derived (n=20) UBE3A KO (n=9) and +CPP (n=8) neurons showing (c) mean frequency and (d) mean amplitude of spontaneous synaptic currents during plasticity induction and 20–30 and 50–60 min post induction. (e) Quantification of frequency of calcium transients from CTR, AS and UBE3A KO lines pictured in f (n>240 for CTR and AS; n>80 for UBE3A KO). *P<0.05, indicates difference for CTR or AS; #for UBE3A KO (Student’s t-test). Early: 20–30 min post induction; late: 45+ min post induction. (f) Example traces of calcium imaging during same protocol as above from (top) CTR, (middle) AS and (bottom) UBE3A KO cultures. Scale bar, 3 min. Error bars represent mean ±s.e.m.
