Figure 4: Directed differentiation of primed and naive hESCs towards neuronal and cardiac lineages.
From: Direct comparison of distinct naive pluripotent states in human embryonic stem cells
(a) Representative images of primed, NCM-, NHSM- and RT-MEF naive hESCs expressing β-TUBULIN when differentiated towards neuronal lineage. β-TUBULIN is expressed higher in neurons derived from primed and RT-MEF naive hESCs compared to NCM- and NHSM-MEF naive hESCs. (b) Representative images of primed, NCM-, NHSM- and RT-MEF naive hESCs expressing mature cardiac markers TROPONIN and MYOSIN when differentiated towards cardiomyocytes. Primed hESCs when differentiated towards cardiac lineage expressing markers TROPONIN and MYOSIN whereas all naive hESCs fail to express the mature markers. Scale bar, 200 μm.
