Figure 2: Evaluation of BLISS sensitivity through genome-wide quantification of SpCas9 on- and off-target DSBs.
From: BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks
(a) On- and off-target sites identified by BLISS, BLESS, GUIDEseq and Digenome-seq. BLISS targets were ranked in descending order based on the number of unique DSB ends aligned to the target per 105 unique BLISS reads. Coloured boxes in the BLESS, GUIDEseq and Digenome-seq columns indicate when the BLISS target was previously found by either of these methods. Individual sites were validated by targeted deep sequencing and the percentage of reads containing an insertion or deletion (indel) is shown. (n=3, error bars show s.e.m.). ON, on-target. OT, off-target. (b) Overlap between on- and off-target sites identified by BLISS versus BLESS. (c) Overlap between on- and off-target sites identified by BLISS versus GUIDEseq and Digenome-seq.
