Figure 2: SFP-1 reacts with sulphide to give a turn-on fluorescence response.
From: Selective fluorescent probes for live-cell monitoring of sulphide
(a–c) Fluorescence spectra of the SFP-1 probe (10 μM) in PBS buffer (10 mM, pH 7.4, 10% CH3CN) at 37 °C for 60 min. Excitation: 300 nm, emission: 310–550 nm. The data represent the average of three independent experiments. (a) Incubated with 50 μM Na2S after 5, 15, 30, 45, 60, 90 and 120 min. (b) Incubated with different concentrations of Na2S (10, 20, 30, 40, 50, 60 and 80 μM). (c) Incubated with various thiols at 1 mM (CYS, cysteine; BME, 2-mercaptoethanol; DTT, dithiothreitol). (d) Fluorescent detection of H2S generation by CBS. Human CBS was mixed with either homocysteine (H) or cysteine (C)+homocysteine (10 mM each). The H2S–producing activity of CBS in the presence of cysteine and homocysteine and 5 μM probe was set at 1 and the data represent the mean±s.d. of at least three independent experiments.
