Figure 1: Intratumoral heterogeneity in primary breast tumours.
From: Single-cell RNA-seq enables comprehensive tumour and immune cell profiling in primary breast cancer
(a) Unsupervised PCA on the transcriptome, indicating a mixed distribution of intra- and interpatient cells. Individual cells are coloured yellow for luminal A, green for luminal B, blue for HER2, and red for TNBC tumours. This colour scheme is maintained throughout the manuscript. (b) Individual cells exhibiting gene expression heterogeneity for ER (ESR1), PR (PGR) and HER2 (ERBB2). The overall single-cell expression profiles agree with the bulk tumour expression profiles and the pathology results. (c) Haematoxylin and eosin staining on formalin-fixed paraffin-embedded slides. Microscopic findings indicated carcinoma and non-carcinoma cells, including tumour-infiltrating lymphocytes9 (TIL, 1–60%). Most of the TNBC tumours except BC10 were heavily infiltrated with lymphocytes, whereas luminal A tumours showed enrichment with carcinoma cells. Scale bar, 100 μm. (d) A part of the tumour tissue in c is magnified to show non-neoplastic cellular components as a representative. Scale bar, 25 μm.
