Figure 5: Comparison of mutant allele frequencies in cfDNA and BM-derived tumour DNA.
From: Circulating tumour DNA sequence analysis as an alternative to multiple myeloma bone marrow aspirates
For 11 subjects with multiple mutations in one or more genes, a scatter plot of cfDNA and BM-derived DNA allele fractions was generated for each mutation in each subject. Linear regression was then assessed for patients with three or more mutations to determine the strength of correlation (R2 value) (a). Comparison of relative tumour and cfDNA AFs and clonal hierarchies determined from cfDNA and BM in two patients, MYL-023 and 058, with three or more mutations in multiple genes (b) and two patients, MYL-020 and 028, with multiple mutations in the same gene (c). AF correlation plot for all cfDNA samples with matching BM samples (n=48) and bar graphs for other samples with multiple mutations excluded from b,c of this figure are presented as Supplementary Figures 14–16.
