Figure 8: Observation of A. castellanii cells expressing ML135-GFP infected by Noumeavirus between 2 and 3 h pi.
From: Noumeavirus replication relies on a transient remote control of the host nucleus
Cells were observed by interference contrast (DIC; scale bar, 10 μm) (a,d,g). DAPI-stained early nucleoids (b, white arrows) and developing viral factories (VF, e,h) produce intense fluorescence masking the DAPI staining at the nucleus (N). GFP staining co-localizes with the viral nucleoids (c, white arrows) and the early VF (f, white arrows). (i) In contrast with the strongly fluorescent nucleus, the mature VF is barely GFP stained.
