Figure 4: T-cell infiltration significantly stratifies patient survival.

(a–f) Survival analysis of all 132 patients based on percentage of cell numbers per patient of all CD3⁺ T cells (a), CD3⁺CD8⁺ cytotoxic T cells (b), CD3⁺CD4⁺Foxp3⁻ Teffs (c), CD3⁺CD4⁺FoxP3⁺ Tregs (d), CD3⁺CD4⁻CD8⁻ other T cells (e) and the ratio of CD4⁺ Teff/Treg per patient (f). N values correspond to uncensored patients (who reached cancer survival end point). In f, patients with no Tregs were excluded (N=3 patients excluded) as no ratio could be calculated. High and low infiltration values were divided based on the median percentage of positive cells or ratio. Significance was determined using the Log-rank Mantel–Cox test. (g–i) Correlation analysis between Treg and CD4⁺ Teff cell counts (g), cytotoxic T cells and CD4⁺ Teff cell counts (h) and cytotoxic T cells and Treg cell counts (i) per core. Pearson correlation coefficient (r) and significance levels (P value) are presented for each correlation. Axis values are shown in log scale for clarity. *P<0.05, **P<0.01, NS, not significant.