Figure 1: Preparation and characterization of siRNA-loaded CaP-rHDL (siRNA-CaP-rHDL). | Nature Communications

Figure 1: Preparation and characterization of siRNA-loaded CaP-rHDL (siRNA-CaP-rHDL).

From: Lipoprotein-biomimetic nanostructure enables efficient targeting delivery of siRNA to Ras-activated glioblastoma cells via macropinocytosis

Figure 1

(a) The outline for the preparation of siRNA-CaP-rHDL. (b) Morphology and particle size of negative control siRNA-loaded CaP-LNC (NC-CaP-LNC) and CaP-rHDL (NC-CaP-rHDL) under a transmission electron microscope after negative staining with sodium phosphotungstate solution (1.75%, w/v). Scale bar, 20 nm. (c) Particle size distribution of NC-CaP-LNC and NC-CaP-rHDL analysed by dynamic light scattering via a Zetasizer. (d) Serum stability of siRNA loaded by NC-CaP-LNC and NC-CaP-rHDL. Naked NC-siRNA, NC-CaP-LNC and NC-CaP-rHDL were dissolved in PBS containing 10% serum and incubated at 37 °C. At different incubation times, aliquots containing 1 μg siRNA was collected, destroyed by lysis buffer to release siRNA and subjected to siRNA detection via agarose gel electrophoresis.

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