Figure 3: KDM3A and KDM3B control the tumorigenic potential of CSC-like cells through Wnt/β-catenin.

(a) The knockdown of KDM3A/B by siRNA in ALDH⁺HCT116 cells. (b) The knockdown of KDM3A/B inhibited the expression level of AXIN2, DKK1, CCND1 and MYC in ALDH⁺HCT116 cells, *P<0.05, **P<0.01, unpaired two-tailed student’s t-test (n=3). (c,d) The Knockdown of KDM3A/B significantly inhibited tumorsphere formation of ALDH⁺HCT116 cells. The number of spheres were counted from five different fields and averaged. The results represent mean±s.d. from three independent experiments. *P<0.05, **P<0.01, unpaired two-tailed Student’s t-test (n=3). (e,f) The knockdown of KDM3A/B significantly inhibited tumorigenic potentials of ALDH⁺HCT116 cells in vivo. Values are mean±s.d. from a total of eight mice. *P<0.05, **P<0.01, unpaired two-tailed Student’s t-test. (g) Comparisons of tumour weights at the end of experiments. *P<0.05, **P<0.01, unpaired two-tailed student’s t-test (n=16).