Figure 3: TAZ and YAP regulate SC proliferation in vivo and in vitro. | Nature Communications

Figure 3: TAZ and YAP regulate SC proliferation in vivo and in vitro.

From: A reciprocal regulatory loop between TAZ/YAP and G-protein Gαs regulates Schwann cell proliferation and myelination

Figure 3

(a) Immunofluorescence labelling for SOX10 (red) and Ki67 (green) in sciatic nerves of wild-type control and TazYapdcKO mice at P7. Nuclei were counterstained with DAPI. Scale bar, 50 μm. (b,c) Quantification of (b) Ki67+ and (c) BrdU+ cells among SOX10+ SCs in sciatic nerves of control and TazYapdcKO mice at P1 and P7 (n=3 animals/genotype; the experiment was repeated on 3 different sections/animal). Data are means±s.e.m. ***P<0.001; Two-tailed unpaired Student’s t-test. (d) Immunofluorescence labelling for BrdU (red) and SOX10 (green) in SCs transfected with control siRNA or siRNAs targeting Taz, Yap, or both. Scale bar, 50 μm. (e) Quantification of proliferation of SCs transfected with control siRNA or siRNA targeting Taz, Yap, or both (n=5 independent experiments). Data plotted are means±s.e.m. *P<0.05, **P<0.01, ***P<0.001; one-way ANOVA with Tukey’s multiple-comparison test. (f) Immunofluorescence labelling for Ki67 (red) in SCs transfected with control vector (GFP only) or Taz-4SA or Yap-S112A expressing vectors. Scale bar, 20 μm. (g) Quantification of Ki67+ expression in SCs transfected with control vector (GFP only) or Taz-4SA or Yap-S112A expressing vectors. The experiment was repeated 5 times. Data are means±s.e.m. **P<0.01, ***P<0.001; one-way ANOVA with Tukey's multiple-comparison test.

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