Figure 2: An epithelial–mesenchymal–epithelial transition during the hepatic differentiation of hESCs.

(a) Immunofluorescence staining of EMT and cell fate markers at the indicated differentiation stages. Scale bar, 20 μm. (b,c) Migration assays for hESC and DE cells. Scale bar, 100 μm. (Data represent mean±s.d. from three technical repeats. ***P<0.001 when compared to D0 using Student’s unpaired t-test).