Figure 3: Single cell qPCR analysis reveals a synchronous EMT during the differentiation of hESCs to DE. | Nature Communications

Figure 3: Single cell qPCR analysis reveals a synchronous EMT during the differentiation of hESCs to DE.

From: A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes

Figure 3

(a) Heatmap of the expressions of selected genes. A complete list of genes used in the study can be found at Supplementary Table 3. (b) Relational network plots. Different colours indicate specific days of treatment, node sizes are 2(relative expression). Open arrows indicate a population of day 5/7 hESC-like cells expressing POU5F1, SOX2, NANOG. Closed arrows indicate a population of cells simultaneously expressing the pluripotent marker genes POU5F1, SOX2, NANOG and the DE markers SOX17, GATA4 and GATA6. (c) Correlation of gene expression for the indicated genes for days 0 through 3. (d) Scatter plots of single cell gene expression for SOX17 and GATA6 versus the epithelial marker gene CDH1 and the mesenchymal marker gene CDH2. Colours are the same as in b.

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