Figure 5: Analysis of the role of EMT-related transcriptional factors in hepatic differentiation of hESCs. | Nature Communications

Figure 5: Analysis of the role of EMT-related transcriptional factors in hepatic differentiation of hESCs.

From: A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes

Figure 5

(a) qRT-PCR analysis of the expression of pluripotency genes in wild-type (WT) and SNAI1/2 and ZEB1/2 knockout cell lines. Significance between WT and mutants were determined using Student’s unpaired t-test (*P<0.05). (b) qRT-PCR analysis of the expression of DE markers in WT and mutant hESC lines. Data represent mean±s.d. from three biological repeats in a,b. Significance between WT and mutants were determined using Student’s unpaired t-test (*P<0.05; **P<0.01; ***P<0.001). (c) Immunofluorescence staining analyses for the hepatic differentiation of hESC lines. Scale bar, 20 μm.

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