Figure 6: SNAI1 mediates Activin A-induced EMT and DE formation. | Nature Communications

Figure 6: SNAI1 mediates Activin A-induced EMT and DE formation.

From: A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes

Figure 6

(a) qRT-PCR analysis of EMT and lineage genes in WT and SNAI1 knockout lines. Data represent mean±s.d. from three biological repeats. Significance between WT and mutants were determined by Student’s unpaired t-test (*P<0.05; **P<0.01; ***P<0.001). (b) Immunofluorescence staining of WT and SNAI1 knockout hESCs at D3. Scale bar, 20 μm. (c,d) Migration assays for WT and SNAI1 knockout lines at D3. Scale bar, 100 μm. Significance between WT and mutant was determined by Student’s unpaired t-test (data represent mean±s.d. from three technical repeats,***P<0.001).

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