Figure 8: SNAI1 is not required for the neural differentiation of hESCs.

(a) qRT-PCR analysis of pluripotency and neural lineage marker in WT and SNAI1 knockout lines after 5 days of 2i treatment. Data represent mean±s.d. from three biological repeats. Significance between WT and mutants were determined by Student’s unpaired t-test (*P<0.05, **P<0.01; ***P<0.001). (b) Immunofluorescence staining of WT and SNAI1 knockout hESCs after 2i treatment. Scale bar, 20 μm.