Figure 4: Screening of known and novel HDAC inhibitors for small molecules modulating NLG1-induced synaptogenesis using synapse microarrays.
From: Synapse microarray identification of small molecules that enhance synaptogenesis
(a) Four structural classes (hydroxamic acids, benzamides, ketones and carboxylic acids) of known HDAC inhibitors are tested. (b) Novel hydroxamic acid containing HDAC inhibitors with varying linker lengths and capping elements. (c) Fold increase in total synapsin intensity upon treatment of cultures with the indicated compounds at three different concentrations (0.1 ÎĽM, blue; 1 ÎĽM, red; and 10 ÎĽM, green). A compound is considered 'active' if it induces an at least 1.5-fold increase in total synapsin intensity over DMSO control level at tested concentrations. While a number of novel hydroxamic acid-containing compounds increased synaptogenesis at 10 ÎĽM concentration, only CN13 (synapsinostat) is active at 1 ÎĽM.
