Figure 6: Prkca gene deletion restores cellular homeostasis and prevents apoptosis. | Nature Communications

Figure 6: Prkca gene deletion restores cellular homeostasis and prevents apoptosis.

From: Protein kinase C-alpha suppresses autophagy and induces neural tube defects via miR-129-2 in diabetic pregnancy

Figure 6

Protein abundance of phosphorylated (p-) PERK and p-IRE1α (a); p-eIF2α and CHOP (b); cleaved caspase 8 (c) and caspase 3 (d) in E8.75 embryos. Bar graphs for protein abundance were quantitative data from three independent experiments. Representative images of the TUNEL assays in E8.75 embryos (e). Apoptotic cells were labelled in red and nuclei were labelled in blue by DAPI. The dense blue V shape areas are the neural plate. The level of the body axis was at hindbrain level. The bar graph showed the quantification of apoptotic cell number. Experiments were repeated using five embryos (N=5) from different dams and five images were obtained from each embryo. Scale bars=70 μm. DM, diabetic mellitus; ND, nondiabetic; WT, wild-type;. *indicates significant difference compared with other groups in one-way ANOVA followed by Tukey tests. P<0.05.

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