Figure 2: Validation of cDNA-smMIPs using on lymphoblastoid cell lines.
From: Quantification of differential gene expression by multiplexed targeted resequencing of cDNA
(a) Quantification of relative gene expresssion on endogeneous transcripts of EBV-transformed lymphoblastoid cell lines compared with average gene expression from RNA-Seq data of 660 samples in the Geuvadis project15 for the same cell type. Two experiments were performed (exp. 1 and exp. 2); in the second experiment, two technical replicates were created by two independent experimenters (designated by Rep. 1 and Rep. 2, see Supplementary Table 2). cDNA for experiments 1 and 2 was generated independently from the same RNA for EBV2 and EBV3. (b) Concordance of differential expression between sample EBV2 and EBV3 for individual smMIPs (N=95).
