Figure 5: Estimation of allelic ratios with cDNA-smMIPs.
From: Quantification of differential gene expression by multiplexed targeted resequencing of cDNA
Concordance of allelic ratios estimated from distinct smMIPs (respectively non-overlapping extension probes and non-overlapping ligation probes) targeting the same SNP in a serial dilution of cDNA from K562 cell line with cDNA from HEK293 cell line (8 dilution steps). For all SNPs, the two cell lines are homozygous for the opposite allele. Reported variation in R2 is s.d.
