Figure 5: Epitope mapping for neutralizing mHKUS-2 and mHKUS-3 antibodies in 1A-S310-677aa. | Nature Communications

Figure 5: Epitope mapping for neutralizing mHKUS-2 and mHKUS-3 antibodies in 1A-S310-677aa.

From: Crystal structure of the receptor binding domain of the spike glycoprotein of human betacoronavirus HKU1

Figure 5

(a, left) Structure of 1A-S310-677aa; middle, schematic diagram of different 1A/B CTD chimeras; right, binding of different mHKUS antibodies to 1A/B chimeric proteins measured by ELISA. +++, positive signal with antibody concentration <10 ng ml−1 ++, positive signal with antibody concentration 10–100 ng ml−1; +, positive signal with antibody concentration 100–1,000 ng ml−1; +/−, positive signal with antibody concentration >1,000 ng ml−1; -, no signal with antibody concentration at 10 μg ml−1. The amino acid residue numbers are from S protein of genotype A HKU1 virus. Experiments were done twice. (b) ELISAs were performed using purified proteins and purified antibodies. The experiments were done in triplicate and the s.d.‘s (n=3) were shown as error bar. The experiments were performed twice and one representative is shown.

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