Figure 6: Inhibition of HKU1 virus entry by mutant 1A-S310-677aa proteins. | Nature Communications

Figure 6: Inhibition of HKU1 virus entry by mutant 1A-S310-677aa proteins.

From: Crystal structure of the receptor binding domain of the spike glycoprotein of human betacoronavirus HKU1

Figure 6

(a) The purified mutant proteins were assayed for their affinity to monoclonal antibodies mHKUS-2, mHKUS-4, and mHKUS-6 by ELISA. Differentiated HTBE cells were incubated with 20 μM of 1A-S310-677aa, 1B-S310-676aaor mutant CTD proteins at 37 °C for 1 h. HKU1 viruses were diluted into the same amount of proteins and added onto the HTBE cells for 4 h. The released viruses from apical wash at 24 h post inoculation were analysed using real-time PCR (b), and cells were fixed and stained with polyclonal rabbit anti HKU1 S antibodies 1814 at a dilution of 1:100 at 48 h post inoculation (c). Scale bar, 50 μm. The amount of HKU1 viral RNA in the BSA control was set as 100%. The experiments were done in triplicate, and the s.d.‘s (n=3) were shown as error bar. The experiments were performed twice and one representative is shown.

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