Figure 4: GluN1–M4 is necessary for ND2 co-localization.
From: An evolutionary switch in ND2 enables Src kinase regulation of NMDA receptors
(a) Cartoon representation of GluN1 and subsequent deletion constructs. (b) Representative images of HEK293 cells expressing GFP-ND2+GluN1, GluN1ΔCTD, GluN1ΔATDΔCTD or GluN1ΔATDΔM4ΔCTD. (c) Cumulative frequency distribution of thresholded PCC values for GFP-ND2 with GluN1, (mean PCC=0.67±0.02; n=82), GluN1ΔCTD (0.65±0.03; n=42), GluN1ΔATDΔCTD (0.61±0.03; n=21), and GluN1ΔATDΔM4ΔCTD (0.09±0.04; n=37). (d) Cumulative frequency distribution of thresholded PCC values for GFP-ND2 with GluN1, (mean PCC=0.82±0.02; n=23), GluN1 M4 (0.12±0.06; n=11), GluN2AN1M4 (0.17±0.06; n=15), GluN1N2AM4 (0.83±0.03; n=21), GluN1AChr M4 (0.80±0.02; n=28), GluN1N/A M4 (0.78±0.03; n=32) and GluN1A/N M4 (0.80±0.02; n=29). Scale bar, 3 μm. Statistically significant differences between populations are indicated by the symbol ‘****’(P<0.0001), and were evaluated by Kruskal–Wallis non-parametric analysis of variance with Dunn’s multiple post hoc comparison tests. n=# of cells. Results are presented as mean±s.e.m. See also Supplementary Figs 4–6 and Supplementary Table 1.
